Synergistic Ca 2 Responses by G i - and G q - coupled G - protein - coupled Receptors Require a Single PLC Isoform
نویسندگان
چکیده
Robert A. Rebres, Tamara I. A. Roach, Iain D. C. Fraser, Finly Philip, Christina Moon, Keng-Mean Lin, Jamie Liu, Leah Santat, Lucas Cheadle, Elliott M. Ross, Melvin I. Simon, and William E. Seaman 4 From the Alliance for Cellular Signaling, Northern California Institute for Research and Education and Department of Medicine, University of California, Veterans Affairs Medical Center, San Francisco, California 94121, the Division of Biology, California Institute of Technology, Pasadena, California 91125, and the Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390
منابع مشابه
Coincidence detection at the level of phospholipase C activation mediated by the m4 muscarinic acetylcholine receptor
BACKGROUND One of the principal mechanisms by which G-protein-coupled receptors evoke cellular responses is through the activation of phospholipase C (PLC) and the subsequent release of Ca2+ from intracellular stores. Receptors that couple to pertussis toxin (PTX)-insensitive G proteins typically evoke large increases in PLC activity and intracellular Ca2+ release. In contrast, receptors that u...
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Alteration in [Ca(2+)](i) (the intracellular concentration of Ca(2+)) is a key regulator of many cellular processes. To allow precise regulation of [Ca(2+)](i) and a diversity of signalling by this ion, cells possess many mechanisms by which they are able to control [Ca(2+)](i) both globally and at the subcellular level. Among these are many members of the superfamily of GPCRs (G-protein-couple...
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Robert A. Rebres, Tamara I. A. Roach, Iain D. C. Fraser, Finly Philip, Christina Moon, Keng-Mean Lin, Jamie Liu, Leah Santat, Lucas Cheadle, Elliott M. Ross, Melvin I. Simon, and William E. Seaman 4 From the Alliance for Cellular Signaling, Northern California Institute for Research and Education and Department of Medicine, University of California, Veterans Affairs Medical Center, San Francisc...
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G protein-coupled receptors (GPCRs) mediate responses to external stimuli in various cell types. Early events, such as the binding of ligand and G proteins to the receptor, nucleotide exchange (NX), and GTPase activity at the Galpha subunit, are common for many different GPCRs. For G(q)-coupled M(1) muscarinic (acetylcholine) receptors (M(1)Rs), we recently measured time courses of intermediate...
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